A thermophilic esterase with high activity towards short- to medium-chain p-nitrophenyl carboxylic esters with optimal activity towards the valerate ester. The AF-Est2 has good solvent and pH stability and is very thermostable, showing no loss of activity after incubation for 30min at 80 C. The structure reveals a bound CoA molecule in the vicinity of the active site which regulates substrate specificity
Archaeoglobus fulgidus thermophilic esterase AF1537