Mutation Report for: Y337A_human-ACHE
| Y337A_human-ACHE | Gene_locus | human-ACHE |
|---|
| Torpedo_number | 330 |
| AA_Change | Y337A |
| Mode_of_mutation | Site directed mutagenesis |
| Modification | Signal transduction |
| | Substrate inhibition |
| | Oxime interaction |
| | Acyl specificity |
| Summary (5) |
| Paper (10) |
| Kinetic_parameter (12) |
| Comment | p.Y337A Tyr337Ala (p.Y368A Tyr368Ala in primary sequence with 31 amino-acids signal peptide);Decrease in catalytic and apparent bimolecular constant Substrate inhibition;absence of substrate inhibition; Acyl specificity;replacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHE; Oxime interaction; reactivation of tabun-inhibited human AChE. Unable to facilitate HI-6-mediated reactivation of tabun-hAChE; The phosphoramidated-hAChE choline-binding site mutant Y337A showed three-times enhanced reactivation capacity with non-triazole imidazole containing aldoximes than with 2PAM |
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