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Mutation Report for: V132K_human-ACHE

Name Class
V132K_human-ACHEGene_locushuman-ACHE
Torpedo_number129
AA_ChangeV132K
Mode_of_mutationSite directed mutagenesis
ModificationBack door hypothesis
SummaryBack door hypothesis;V132K introduce a charge and should block the door, 6X increase of Km for ATc but not for TB through interaction with W86;Kronman_1994_J.Biol.Chem_269_27819
PaperKronman_1994_J.Biol.Chem_269_27819
Kinetic_parameter3,3-dimethylbutylthioacetate_V132K_human-ACHE
Acetylthiocholine_V132K_human-ACHE
Edrophonium_V132K_human-ACHE
Commentp.V132K Val132Lys (p.V163K Val163Lys in primary sequence with 31 amino-acids signal peptide) Back door hypothesis;V132K introduce a charge and should block the door, 6X increase of Km for ATc but not for TB through interaction with W86

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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