Mutation Report for: T284N/P285K/L286S/S287N_human-BCHE

T284N/P285K/L286S/S287N_human-BCHE	Gene_locus	human-BCHE
	Torpedo_number (5)
	AA_Change	T284N/P285K/L286S/S287N
	Mode_of_mutation	Site directed mutagenesis
	Modification	Search for phosphotriesterase activity
		OP-resistance
	Summary	Search for phosphotriesterase activity;;Terekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
	Paper	Terekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
	Comment	p.T284N/P285K/L286S/S287N Thr284Asn/Pro285Lys/Leu286Ser/Ser287Asn (p.T312N/P313K/L314S/S315N Thr312Asn/Pro313Lys/Leu314Ser/Ser315Asn in primary sequence with 28 amino-acids signal peptide) Multiple amino acid substitutions in the acyl-binding loop 284-TPLSV-288 284-ThrProLeuSerVal-288 close to the active site. replaced by ProProLeuArgSer QSIHI clone 13. Slow self-reactivation after inhibition by paraoxon

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

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