p.T250L Thr250Leu (p.T278L Thr278Leu in primary sequence with 28 amino-acids signal peptide) Mutation designed by -Protein Repair One Stop Shop- process for the production of active human butyrylcholinesterase in a prokaryotic system. The mutation gives Leu as in ACHE of Torpedo Californica and Bungarus fasciatus
