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Mutation Report for: K23T_human-ACHE

Name Class
K23T_human-ACHEGene_locushuman-ACHE
Torpedo_number21
AA_ChangeK23T
Mode_of_mutationSite directed mutagenesis
ModificationExpression in E Coli
PROSS algorithm
SummaryPROSS algorithm;Expression in E Coli;Goldenzweig_2016_Mol.Cell_63_337
PaperGoldenzweig_2016_Mol.Cell_63_337
Structure5HQ3
Commentp.K23T Lys23Thr (p.K54T Lys54Thr in primary sequence with 31 amino-acids signal peptide) mutation designed by -Protein Repair One Stop Shop- process for the production of active human acetylcholinesterase in a prokaryotic system. The mutation gives Thr as in human BCHE and drosophila ACHE

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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