Mutation Report for: F338A_human-ACHE

F338A_human-ACHE	Gene_locus	human-ACHE
	Torpedo_number	331
	AA_Change	F338A
	Mode_of_mutation	Site directed mutagenesis
	Modification	Hydrophobic subsite
		Acyl specificity
		Signal transduction
	Summary	Signal transduction; reduced kcat, marginal effect on inhibition by edrophonium;Shafferman_1992_EMBO.J_11_3561;Shafferman_1992_4th.ChE.Meeting.Eilat__165;Ordentlich_1993_J.Biol.Chem_268_17083;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Barak_1994_J.Biol.Chem_269_6296;Shafferman_1995_5th.ChE.Meeting.Madras__189;Shafferman_1996_Biochem.J_318_833
		Acyl specificity;Acyl pocket decreased catalytic activity, orientation of His 447;Barak_2002_Biochemistry_41_8245;Shafferman_2005_Chem.Biol.Interact_157-158_123
		Oxime interaction;;Cochran_2011_J.Biol.Chem_286_29718
	Paper (12)
	Kinetic_parameter (11)
	Comment	p.F338A Phe338Ala (p.F369A Phe369Ala in primary sequence with 31 amino-acids signal peptide) Signal transduction: reduced kcat, marginal effect on inhibition by edrophonium; Acyl pocket decreased catalytic activity, orientation of His 447

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

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