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Mutation Report for: F338A_human-ACHE

Name Class
F338A_human-ACHEGene_locushuman-ACHE
Torpedo_number331
AA_ChangeF338A
Mode_of_mutationSite directed mutagenesis
ModificationHydrophobic subsite
Acyl specificity
Signal transduction
SummarySignal transduction; reduced kcat, marginal effect on inhibition by edrophonium;Shafferman_1992_EMBO.J_11_3561;Shafferman_1992_4th.ChE.Meeting.Eilat__165;Ordentlich_1993_J.Biol.Chem_268_17083;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Barak_1994_J.Biol.Chem_269_6296;Shafferman_1995_5th.ChE.Meeting.Madras__189;Shafferman_1996_Biochem.J_318_833
Acyl specificity;Acyl pocket decreased catalytic activity, orientation of His 447;Barak_2002_Biochemistry_41_8245;Shafferman_2005_Chem.Biol.Interact_157-158_123
Oxime interaction;;Cochran_2011_J.Biol.Chem_286_29718
Paper (12)
Kinetic_parameterEdrophonium_F338A_human-ACHE
Paranitrophenylacetate_F338A_human-ACHE
S-N-Propylthioacetate_F338A_human-ACHE
3,3-dimethylbutylthioacetate_F338A_human-ACHE
Acetylthiocholine_F338A_human-ACHE
Hexamethonium_F338A_human-ACHE
BW284C51_F338A_human-ACHE
Propidium_F338A_human-ACHE
Decamethonium_F338A_human-ACHE
Tacrine_F338A_human-ACHE
HuperzineA_F338A_human-ACHE
Commentp.F338A Phe338Ala (p.F369A Phe369Ala in primary sequence with 31 amino-acids signal peptide) Signal transduction: reduced kcat, marginal effect on inhibition by edrophonium; Acyl pocket decreased catalytic activity, orientation of His 447

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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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