Mutation Report for: F295A_human-ACHE

F295A_human-ACHE	Gene_locus	human-ACHE
	Torpedo_number	288
	AA_Change	F295A
	Mode_of_mutation	Site directed mutagenesis
	Modification	Acyl specificity
	Summary	Acyl specificity;Acyl pocket, OP-specificity.inhanced affinity for BTC;Ordentlich_1993_J.Biol.Chem_268_17083;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Ashani_1994_Mol.Pharmacol_45_555;Barak_1994_J.Biol.Chem_269_6296;Ordentlich_1995_5th.ChE.Meeting.Madras__221;Shafferman_1995_5th.ChE.Meeting.Madras__189
		OP-specificity;increased reactivity to DFP DEFP and paraoxon;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Ashani_1994_Mol.Pharmacol_45_555;Barak_1994_J.Biol.Chem_269_6296;Ordentlich_1995_5th.ChE.Meeting.Madras__221;Shafferman_1995_5th.ChE.Meeting.Madras__189;Ordentlich_1996_J.Biol.Chem_271_11953
		Acyl specificity;Acyl pocket, decreased catalytic activity, orientation of His 447;Barak_2002_Biochemistry_41_8245
		VX stereospecificity;decrease in stereoselectivity;Ordentlich_2004_Biochemistry_43_11255;Ordentlich_2005_Chem.Biol.Interact_157-158_191
	Paper (11)
	Kinetic_parameter (8)
	Comment	p.F295A Phe295Ala (p.F326A Phe326Ala in primary sequence with 31 amino-acids signal peptide) Acyl specificity;Acyl pocket, OP-specificity.inhanced affinity for BTC; increased reactivity to DFP DEFP and paraoxon, decreased catalytic activity, orientation of His 447

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page