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Mutation Report for: F295A_human-ACHE

Name Class
F295A_human-ACHEGene_locushuman-ACHE
Torpedo_number288
AA_ChangeF295A
Mode_of_mutationSite directed mutagenesis
ModificationAcyl specificity
SummaryAcyl specificity;Acyl pocket, OP-specificity.inhanced affinity for BTC;Ordentlich_1993_J.Biol.Chem_268_17083;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Ashani_1994_Mol.Pharmacol_45_555;Barak_1994_J.Biol.Chem_269_6296;Ordentlich_1995_5th.ChE.Meeting.Madras__221;Shafferman_1995_5th.ChE.Meeting.Madras__189
OP-specificity;increased reactivity to DFP DEFP and paraoxon;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Ashani_1994_Mol.Pharmacol_45_555;Barak_1994_J.Biol.Chem_269_6296;Ordentlich_1995_5th.ChE.Meeting.Madras__221;Shafferman_1995_5th.ChE.Meeting.Madras__189;Ordentlich_1996_J.Biol.Chem_271_11953
Acyl specificity;Acyl pocket, decreased catalytic activity, orientation of His 447;Barak_2002_Biochemistry_41_8245
VX stereospecificity;decrease in stereoselectivity;Ordentlich_2004_Biochemistry_43_11255;Ordentlich_2005_Chem.Biol.Interact_157-158_191
Paper (11)
Kinetic_parameterAcetylthiocholine_F295A_human-ACHE
Edrophonium_F295A_human-ACHE
Hexamethonium_F295A_human-ACHE
BW284C51_F295A_human-ACHE
Propidium_F295A_human-ACHE
Decamethonium_F295A_human-ACHE
Tacrine_F295A_human-ACHE
HuperzineA_F295A_human-ACHE
Commentp.F295A Phe295Ala (p.F326A Phe326Ala in primary sequence with 31 amino-acids signal peptide) Acyl specificity;Acyl pocket, OP-specificity.inhanced affinity for BTC; increased reactivity to DFP DEFP and paraoxon, decreased catalytic activity, orientation of His 447

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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