Mutation Report for: E202A_human-ACHE
| E202A_human-ACHE | Gene_locus | human-ACHE |
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| Torpedo_number | 199 |
| AA_Change | E202A |
| Mode_of_mutation | Site directed mutagenesis |
| Modification (4) |
| Summary (5) |
| Paper (8) |
| Kinetic_parameter (7) |
| Comment | p.E202A Glu202Ala (p.E233A Glu233Ala in primary sequence with 31 amino-acids signal peptide) Catalysis;Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation; H-bond network; not inhibited at high substrate concentration; Acylation,Phosphorylation;low effect on inhibition by DFP and DEFP but high for Paraoxon; Aging:E202 contributes to the aging process by stabilizing the imidazolium His447 |
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