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Mutation Report for: E202A_human-ACHE

Name Class
E202A_human-ACHEGene_locushuman-ACHE
Torpedo_number199
AA_ChangeE202A
Mode_of_mutationSite directed mutagenesis
ModificationAcylation,Phosphorylation
Substrate inhibition
Aging
H-bond network
SummaryCatalysis;Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation;Shafferman_1992_EMBO.J_11_3561;Shafferman_1992_4th.ChE.Meeting.Eilat__165;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Shafferman_1995_5th.ChE.Meeting.Madras__189;Shafferman_1996_Biochem.J_318_833
H-bond network;Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation;Shafferman_1992_EMBO.J_11_3561;Shafferman_1992_4th.ChE.Meeting.Eilat__165;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Shafferman_1995_5th.ChE.Meeting.Madras__189;Shafferman_1996_Biochem.J_318_833
Substrate inhibition;E202 mutant not inhibited at high substrate concentration;Shafferman_1992_EMBO.J_11_3561;Shafferman_1992_4th.ChE.Meeting.Eilat__165;Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097;Shafferman_1995_5th.ChE.Meeting.Madras__189;Shafferman_1996_Biochem.J_318_833
Acylation,Phosphorylation;low effect on inhibition by DFP and DEFP but high for Paraoxon;Ordentlich_1995_5th.ChE.Meeting.Madras__221;Ordentlich_1996_J.Biol.Chem_271_11953;Shafferman_1996_Biochem.J_318_833
Aging;E202 contributes to the aging process by stabilizing the imidazolium His447;Shafferman_1996_Biochem.J_318_833
Paper (8)
Kinetic_parameter (7)
Commentp.E202A Glu202Ala (p.E233A Glu233Ala in primary sequence with 31 amino-acids signal peptide) Catalysis;Decrease in catalytic and bimolecular constant, identical effect on charge and uncharged substrate, affects acylation-deacylation steps and not the noncovalent complex formation; H-bond network; not inhibited at high substrate concentration; Acylation,Phosphorylation;low effect on inhibition by DFP and DEFP but high for Paraoxon; Aging:E202 contributes to the aging process by stabilizing the imidazolium His447
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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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