p.D454L Asp454Leu (p.D482L Asp482Leu in primary sequence with 28 amino-acids signal peptide) Mutation designed by -Protein Repair One Stop Shop- process for the production of active human butyrylcholinesterase in a prokaryotic system. The mutation gives Asp as in ACHE of Torpedo californica, human, mouse, and Bungarus fasciatus
