Lactonizing lipase. This family corresponds to family I.1 of the classification of Arpigny and Jaeger (1999) The pseudomonase lipase efficiently catalyzes intramolecular transesterification of omega-hydroxyesters to form macrocyclic lactones. The production of the enzymatically active LipL requires a specific molecular chaperon (LimL protein) together with a low-M(r) lipase-activation-factor (LAF) (reduced form of glutathione) for formation of intermolecular disulfide bound