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Mutation Report for: A328W/Y332A_human-BCHE

Name Class
A328W/Y332A_human-BCHEGene_locushuman-BCHE
Torpedo_number330
334
330,334
AA_ChangeA328W/Y332A
Mode_of_mutationSite directed mutagenesis
ModificationAcyl specificity
Cocaine hydrolysis
SummaryCocaine hydrolysis;CocE 40-fold increased kcat and low increase in Km;Sun_2002_Mol.Pharmacol_62_220
Paper (6)
Commentp.A328W/Y332A Ala328Trp/Tyr332Ala (p.A356W/Y360A Ala328Trp/Tyr332Ala in primary sequence with 28 amino-acids signal peptide) Cocaine hydrolysis;CocE 40-fold increased kcat and low increase in Km

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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