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Torpedo_number Report for: 334

drome-ACHE
MutationKin.TypeEvaluation systemEffectReference
Y412A sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
Y412A sdmBinding of Triton X-100 studies slightly decreased inhibition by triton of maloxon phosphorylationMarcel_2000_J.Biol.Chem_275_11603
Y412+A sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43
Y412del sdmInsecticide sensitivityBoublik_2002_Protein.Eng_15_43

human-ACHE : (kinetic parameters)
MutationKin.TypeEvaluation systemEffectReference
Y341AkinsdmOmega loop interactionNo effectVelan_1996_FEBS.Lett_395_22
Y341AkinsdmPeripheral Anionic Sitelow decrease in affinity for edrophonium, no inhib by excess substrateShafferman_1992_EMBO.J_11_3561,
Shafferman_1992_4th.ChE.Meeting.Eilat__165,
Barak_1994_J.Biol.Chem_269_6296,
Shafferman_1995_5th.ChE.Meeting.Madras__189
Y341Akinsdmoxime interactionreactivation of tabun-inhibited human AChE. The dissociation constant 3.5-fold increasedArtursson_2009_Toxicology_265_108
D74N/
Y341A		 sdmPeripheral Anionic Siteno cooperativity D74 Y341Shafferman_1995_5th.ChE.Meeting.Madras__189
D74N/
W286A/
Y341A		 sdmPeripheral Anionic Sitecooperativity of mutations on D74 W286 for bisquaternary PAS inhibShafferman_1995_5th.ChE.Meeting.Madras__189
W286A/
Y341A		 sdmPeripheral Anionic Sitesynergetic effects on PAS ligands inhibitionShafferman_1995_5th.ChE.Meeting.Madras__189

human-BCHE
MutationKin.TypeEvaluation systemEffectReference
Y332S sdmOP-resistanceincreased resistance to nerve agent retained robust intrinsic catalytic efficiencyDwyer_2014_Biochemistry_53_4476
Y332S/
D340H		 sdmOP-resistanceincreased resistance to nerve agent retained robust intrinsic catalytic efficiencyDwyer_2014_Biochemistry_53_4476
A328W/
Y332A		 sdmCocaine hydrolysisCocE 40-fold increased kcat and low increase in KmSun_2002_Mol.Pharmacol_62_220
A328W/
Y332A/
Y419S		 sdmCocaine hydrolysisno activityHamza_2005_J.Phys.Chem.B_109_4776
A328W/
Y332G		 sdmCocaine hydrolysisCatalytic efficiency slightly higher than A328W/Y332AHamza_2005_J.Phys.Chem_109_4776
A199S/
F227A/
P285A/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 4400-fold improved catalytic efficiency against-cocaine E9 Zheng_2014_Nat.Commun_5_3457
A199S/
F227A/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 2000-fold improved catalytic efficiency against-cocaine E9 Zheng_2008_J.Am.Chem.Soc_130_12148
A199S/
F227A/
S287G/
A328W/
Y332G/
F364C/
M532C		 sdmCocaine hydrolysisimproved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bondsFang_2014_Chem.Biol.Interact_214C_18
A199S/
F227A/
S287G/
A328W/
Y332G/
V377C/
A516C		 sdmCocaine hydrolysisimproved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bondsFang_2014_Chem.Biol.Interact_214C_18
A199S/
F227A/
S287G/
A328W/
Y332G/
N535C		 sdmCocaine hydrolysisimproved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bondsFang_2014_Chem.Biol.Interact_214C_18
A199S/
F227A/
S287G/
A328W/
Y332G/
F329V		 sdmCocaine hydrolysis E11 Zheng_2010_Biochemistry_49_9113
A199S/
F227L/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 1000-fold improved catalytic efficiency against-cocaine E Zheng_2010_Biochemistry_49_9113
A199S/
F227I/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 1000-fold improved catalytic efficiency against-cocaine E Zheng_2010_Biochemistry_49_9113
A199S/
F227V/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 1000-fold improved catalytic efficiency against-cocaine E Zheng_2010_Biochemistry_49_9113
F227A/
S287G/
A328W/
Y332M		 sdmCocaine hydrolysisAME359 CocH, 34-fold improved catalytic efficiency against (-)-cocaine Pancook_2003_FASEB.J_17_A565
A199S/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysisCocH1 456-fold improved catalytic efficiency against (-)-cocaine Pan_2005_Proc.Natl.Acad.Sci.U.S.A_102_16656
A199S/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysisSelectively blocks cocaine toxicity and reinstatement of drug seeking in rats (mutant fused to albumin TV-1380)Brimijoin_2008_Neuropsychopharmacology_33_2715
A199S/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysisYang_2010_Chem.Biol.Interact_187_148
A199S/
S287G/
A328W/
Y332G/
L286I		 sdmCocaine hydrolysisE10Zheng_2010_Biochemistry_49_9113
Y332F sdmSuccinylthiocholine bindingHigher Km and kcatMasson_1997_Biochemistry_36_2266
Y332F sdmPeripheral Anionic siteinterraction 70/332Masson_1999_Biochim.Biophys.Acta_1433_281
Y332A sdmPeripheral Anionic siteinterraction 70/332Masson_1999_Biochim.Biophys.Acta_1433_281
D70G/
Y332A		 sdmPeripheral Anionic siteinterraction 70/332Masson_1999_Biochim.Biophys.Acta_1433_281
D70Y/
Y332D		 sdmPeripheral Anionic siteinterraction 70/332Masson_1999_Biochim.Biophys.Acta_1433_281
Y332D sdmPeripheral Anionic siteinterraction 70/332Masson_1999_Biochim.Biophys.Acta_1433_281

mouse-BCHE
MutationKin.TypeEvaluation systemEffectReference
A199S/
F227A/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis Chen_2015_Biochem.J_466_243

nipbr-ACHEB
MutationKin.TypeEvaluation systemEffectReference
Y349G sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823
Y349L sdmInsecticide sensitivitySchulze_2005_Anal.Chem_77_5823
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