Torpedo_number Report for: 288,290

brafl-ACHE2

Mutation	Kin.	Type	Evaluation system	Effect	Reference
C310A/ F312I		sdm	Inactivation by sulfhydryl		Pezzementi_2006_Invert.Neurosci_6_47

human-ACHE : (kinetic parameters)

Mutation	Kin.	Type	Evaluation system	Effect	Reference
F295L/ F297V	kin	sdm	Acyl specificity	Acyl pocket, Increases hydrolysis of BTC	Ordentlich_1993_J.Biol.Chem_268_17083, Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097, Ashani_1994_Mol.Pharmacol_45_555
F295L/ F297V	kin	sdm	OP-specificity	increases reactivity towards DFP DEFP and paraoxon	Ordentlich_1996_J.Biol.Chem_271_11953'
F295L/ F297V	kin	sdm	OP-specificity	decrease in stereoselectivity	Ordentlich_2004_Biochemistry_43_11255
F295L/ F297V	kin	sdm	Acyl specificity	replacement of aromatic active center residues in human-ACHE by the corresponding residues in human-BCHE	Kaplan_2001_Biochemistry_40_7433
F295A/ F297A		sdm	Acyl specificity	VX stereospecificity decrease in stereoselectivity	Ordentlich_2005_Chem.Biol.Interact_157-158_191

mouse-ACHE : (kinetic parameters)

Mutation	Kin.	Type	Evaluation system	Effect	Reference
F295L/ F297I	kin	sdm	Acyl specificity	Acyl pocket, reduced catalytic activity for ACT and enhanced for BCT	Vellom_1993_Biochemistry_32_12

nipbr-ACHEB

Mutation	Kin.	Type	Evaluation system	Effect	Reference
M301A/ W303A		sdm	Insecticide sensitivity		Schulze_2005_Anal.Chem_77_5823
M301A/ W303L		sdm	Insecticide sensitivity		Schulze_2005_Anal.Chem_77_5823

torma-ACHE

Mutation	Kin.	Type	Evaluation system	Effect	Reference
F288L/ F290V		sdm	Acyl specificity	Acyl pocket hydrolysis of BTC as well as ATC , inhibited also by iso-OMPA	Harel_1992_Proc.Natl.Acad.Sci.U.S.A_89_10827

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page