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Torpedo_number
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Torpedo_number Report for: 285
bunfa-ACHE
:
(
kinetic parameters
)
Mutation
Kin.
Type
Evaluation system
Effect
Reference
M70Y/
K285D
kin
sdm
Peripheral Anionic Site
Decrease Ki of PAS ligands
Cousin_1996_J.Biol.Chem_271_15099
M70Y/
K285D
kin
sdm
Peripheral Anionic Site
Decrease Ki for PAS ligands
Cousin_1996_J.Biol.Chem_271_15099
K285D
kin
sdm
Peripheral Anionic Site
Decrease Ki for PAS ligands
Cousin_1996_J.Biol.Chem_271_15099
human-ACHE
:
(
kinetic parameters
)
Mutation
Kin.
Type
Evaluation system
Effect
Reference
E84Q/
E292A
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A/
D349N/
E358Q
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E285A/
E292A/
D349N/
E358Q
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E84Q/
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Heat and pressure stability
strengthened heat stability decreased pressure stability
Clery-Barraud_2002_Eur.J.Biochem_269_4297
E84Q/
E285A/
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesisno significant effect
Shafferman_1994_EMBO.J_13_3448
E292A
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E292A/
D349N/
E358Q
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E292A/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
E292A/
D349N/
E358Q/
E389Q/
D390N
kin
sdm
Electrostatic
Electrostatic attraction hypothesis no significant effect
Shafferman_1994_EMBO.J_13_3448
human-BCHE
Mutation
Kin.
Type
Evaluation system
Effect
Reference
G283S
sdm
PROSS algorithm
Expression in E Coli
Brazzolotto_2017_Molecules_22_
YGTP282-285NHML
sdm
Search for phosphotriesterase activity
Phosphotriesterase activity Hydrolysis of OP
McGarry_2020_Toxicol.Sci_174_133
PYGTP281-284EPYNGTA/
G117H
sdm
Search for phosphotriesterase activity
Phosphotriesterase activity Hydrolysis of OP
Hester_2019_Chem.Res.Toxicol_32_1801
A277W/
G283D
sdm
Peripheral Anionic Site
A277 G283 play no role in catalytic activity
Masson_1995_5th.ChE.Meeting.Madras__230
,
Masson_1996_Eur.J.Biochem_235_36
G283D
sdm
Peripheral Anionic Site
behave like wild type
Masson_1995_5th.ChE.Meeting.Madras__230
,
Masson_1996_Eur.J.Biochem_235_36
mouse-ACHE
Mutation
Kin.
Type
Evaluation system
Effect
Reference
E292Q
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
E84Q/
E91Q/
D280V/
D283N/
E292Q/
D372N
sdm
Electrostatic
electrostatic influence reduced, influences FAS2 inhibition and its ionic strength dependance
Radic_1997_J.Biol.Chem_272_23265
ratno-ACHE
Mutation
Kin.
Type
Evaluation system
Effect
Reference
PQES290-293SG
sdm
Acyl specificity
less specific more inhibition by OP and Carbamates
Pleiss_1999_J.Mol.Catal.B.Enzym_6_287
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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