Torpedo_number Report for: 278

human-ACHE: (kinetic parameters)

Mutation	Kin.	Type	Evaluation system	Effect	Reference
Y72A/ E285A	kin	sdm	Peripheral Anionic Site	Ki of PAS ligand increased >10X	Barak_1994_J.Biol.Chem_269_6296, Shafferman_1995_5th.ChE.Meeting.Madras__189
Y72A/ E285A/ W286A	kin	sdm	Peripheral Anionic Site	Ki of PAS ligand increased up to 400X	Barak_1994_J.Biol.Chem_269_6296, Shafferman_1995_5th.ChE.Meeting.Madras__189
E84Q/ E285A/ E292A/ D349N/ E358Q	kin	sdm	Electrostatic	Electrostatic attraction hypothesis no significant effect	Shafferman_1994_EMBO.J_13_3448
E84Q/ E285A/ E292A/ D349N/ E358Q/ E389Q/ D390N	kin	sdm	Electrostatic	Electrostatic attraction hypothesisno significant effect	Shafferman_1994_EMBO.J_13_3448
Y124A/ E285A/ W286A	kin	sdm	Peripheral Anionic Site	low effect on Km Kcat but 400 increase of Ki for PAS inhib	Barak_1994_J.Biol.Chem_269_6296, Shafferman_1995_5th.ChE.Meeting.Madras__189
E285A	kin	sdm	Peripheral Anionic Site	2 to 10 fold increase of inhibition K for PAS ligands	Barak_1994_J.Biol.Chem_269_6296, Shafferman_1994_EMBO.J_13_3448, Shafferman_1995_5th.ChE.Meeting.Madras__189
E285A/ W286A	kin	sdm	Peripheral Anionic Site	10 to 200fold increase of inhibition K for PAS ligands	Barak_1994_J.Biol.Chem_269_6296, Shafferman_1995_5th.ChE.Meeting.Madras__189

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page