Torpedo_number
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Torpedo_number Report for: 289

human-BCHE
MutationKin.TypeEvaluation systemEffectReference
A199S/
F227A/
P285A/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 4400-fold improved catalytic efficiency against-cocaine E9 Zheng_2014_Nat.Commun_5_3457
A199S/
F227A/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 2000-fold improved catalytic efficiency against-cocaine E9 Zheng_2008_J.Am.Chem.Soc_130_12148
A199S/
F227A/
S287G/
A328W/
Y332G/
F364C/
M532C		 sdmCocaine hydrolysisimproved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bondsFang_2014_Chem.Biol.Interact_214C_18
A199S/
F227A/
S287G/
A328W/
Y332G/
V377C/
A516C		 sdmCocaine hydrolysisimproved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bondsFang_2014_Chem.Biol.Interact_214C_18
A199S/
F227A/
S287G/
A328W/
Y332G/
N535C		 sdmCocaine hydrolysisimproved catalytic efficiency against-cocaine and increased biological half-life with new cross-subunit SS bondsFang_2014_Chem.Biol.Interact_214C_18
A199S/
F227A/
S287G/
A328W/
Y332G/
F329V		 sdmCocaine hydrolysis E11 Zheng_2010_Biochemistry_49_9113
A199S/
F227L/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 1000-fold improved catalytic efficiency against-cocaine E Zheng_2010_Biochemistry_49_9113
A199S/
F227A/
S287G/
A328W/
E441D		 sdmCocaine hydrolysis improved catalytic efficiency against-cocaine E Xue_2011_Mol.Pharmacol_79_290
A199S/
F227I/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 1000-fold improved catalytic efficiency against-cocaine E Zheng_2010_Biochemistry_49_9113
A199S/
F227V/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis 1000-fold improved catalytic efficiency against-cocaine E Zheng_2010_Biochemistry_49_9113
F227A/
S287G/
A328W/
Y332M		 sdmCocaine hydrolysisAME359 CocH, 34-fold improved catalytic efficiency against (-)-cocaine Pancook_2003_FASEB.J_17_A565
A199S/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysisCocH1 456-fold improved catalytic efficiency against (-)-cocaine Pan_2005_Proc.Natl.Acad.Sci.U.S.A_102_16656
A199S/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysisSelectively blocks cocaine toxicity and reinstatement of drug seeking in rats (mutant fused to albumin TV-1380)Brimijoin_2008_Neuropsychopharmacology_33_2715
A199S/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysisYang_2010_Chem.Biol.Interact_187_148
A199S/
S287G/
A328W/
Y332G/
L286I		 sdmCocaine hydrolysisE10Zheng_2010_Biochemistry_49_9113
T284P/
S287R/
V288S		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284Q/
P285S/
L286I/
S287H/
V288I		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284H/
P285T/
L286I/
S287H/
V288G		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550
T284N/
P285K/
L286S/
S287N		 sdmSearch for phosphotriesterase activityTerekhov_2017_Proc.Natl.Acad.Sci.U.S.A_114_2550

mouse-ACHE : (kinetic parameters)
MutationKin.TypeEvaluation systemEffectReference
F295L/
R296S/
F297I		 sdmAcyl specificityAcyl pocket reduced catalytic activity for ACT and enhanced for BCTVellom_1993_Biochemistry_32_12
R296SkinsdmAcyl pocketslightly enhances BTC hydrolysisVellom_1993_Biochemistry_32_12

mouse-BCHE
MutationKin.TypeEvaluation systemEffectReference
A199S/
F227A/
S287G/
A328W/
Y332G		 sdmCocaine hydrolysis Chen_2015_Biochem.J_466_243
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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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