Torpedo_number Report for: 130

drome-ACHE

Mutation	Kin.	Type	Evaluation system	Effect	Reference
Y200A		sdm	Insecticide sensitivity		Boublik_2002_Protein.Eng_15_43

human-ACHE : (kinetic parameters)

Mutation	Kin.	Type	Evaluation system	Effect	Reference
Y133A	kin	sdm	Choline binding site	Catalysis/Maintenance of Choline binding site 90 fold increase of Km for ATCh 760 fold red of kapp	Ordentlich_1995_J.Biol.Chem_270_2082
Y133F	kin	sdm	Choline binding site	Catalysis/Maintenance of Choline binding site 7 fold less efficient (kapp)/WT, 30 fold decrease in affinity for edrophonium	Ordentlich_1995_J.Biol.Chem_270_2082

human-BCHE

Mutation	Kin.	Type	Evaluation system	Effect	Reference
Y128C		nat	Silent variant	Natural mutation Silent phenotype,extremely low BChE activity	Hidaka_1997_Am.Hum.Genet_61_491

luccu-E3aest7

Mutation	Type	Evaluation system	Effect	Reference
Y148F	sdm	OP-resistance	search for OP hydrolase and MCE activities	Heidari_2004_Insect.Biochem.Mol.Biol_34_353
Y148F	sdm	Pyrethroids hydrolysis	search for Pyrethroids hydrolysis activities	Heidari_2005_Insect.Biochem.Mol.Biol_35_597
Y148F	sdm	Pyrethroids hydrolysis	Hydrolysis of individual isomers of fluorogenic pyrethroid analogs by mutant carboxylesterases	Devonshire_2007_Insect.Biochem.Mol.Biol_37_891
G137D/ Y148F	sdm	OP-resistance	search for OP hydrolase and MCE activities	Heidari_2004_Insect.Biochem.Mol.Biol_34_353
G137D/ Y148F	sdm	Pyrethroids hydrolysis	search for Pyrethroids hydrolysis activities	Heidari_2005_Insect.Biochem.Mol.Biol_35_597
G137D/ Y148F	sdm	Pyrethroids hydrolysis	Hydrolysis of individual isomers of fluorogenic pyrethroid analogs by mutant carboxylesterases	Devonshire_2007_Insect.Biochem.Mol.Biol_37_891

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page