Substrate Report for: DTNB

This chemiluminescent probe is not substrate but react with a product of reaction of a substrate hydrolyzed by an enzyme: this compound reacts with thiocholine after hydrolysis of acetylthiocholine by cholinesterases. A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate

General

Type		Chemiluminescent Probe
Chemical_Nomenclature		5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid
Canonical SMILES		C1=CC(=C(C=C1SSC2=CC(=C(C=C2)[N+](=O)[O-])C(=O)O)C(=O)O)[N+](=O)[O-]
InChI		InChI=1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
InChIKey		KIUMMUBSPKGMOY-UHFFFAOYSA-N
Other name(s)		5,5'-Dithiobis(2-nitrobenzoic acid) ; Ellman's Reagent ; 3-Carboxy-4-nitrophenyl disulfide ; CHEMBL395814 ; CHEBI:86228 ; SCHEMBL26775 ; ZINC2041301

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MW	\|	396.4
Formula	\|	C14H8N2O8S2
CAS_number	\|	69-78-3
PubChem	\|	6254
UniChem	\|	KIUMMUBSPKGMOY-UHFFFAOYSA-N
IUPHAR	\|
Wikipedia	\|

Target

References:

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Title:

Ellman GL, Lysko H

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Title:

Ellman GL, Sullivan CV

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Title:

Ellman GL, Courtney KD, Andres V, Featherstone RM, Andres V, Jr.

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PubMed