Structure Report for: 7XDD

Huang, S., Jia, A., Song, W., Hessler, G., Meng, Y., Sun, Y., Xu, L., Laessle, H., Jirschitzka, J., Ma, S., Xiao, Y., Yu, D., Hou, J., Liu, R., Sun, H., Liu, X., Han, Z., Chang, J., Parker, J., Chai, J.

Title: Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity
Huang S, Jia A, Song W, Hessler G, Meng Y, Sun Y, Xu L, Laessle H, Jirschitzka J and Chai J <10 more author(s)> Huang S, Jia A, Song W, Hessler G, Meng Y, Sun Y, Xu L, Laessle H, Jirschitzka J, Ma S, Xiao Y, Yu D, Hou J, Liu R, Sun H, Liu X, Han Z, Chang J, Parker JE, Chai J (- 10)
Ref: Science, :, 2022 : PubMed
Abstract


ESTHER: Huang_2022_Science__
PubMedSearch: Huang 2022 Science
Gene_locus related to this paper: arath-eds1, arath-PAD4

Abstract

Plant nucleotide-binding leucine-rich repeat-containing (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain sense pathogen effectors to enable TIR-encoded NADase activity for immune signaling. TIR-NLR signaling requires helper NLRs N requirement gene 1 (NRG1) and Activated Disease Resistance 1 (ADR1), and Enhanced Disease Susceptibility 1 (EDS1) that forms a heterodimer with each of its paralogs Phytoalexin Deficient 4 (PAD4) and Senescence-Associated Gene101 (SAG101). Here, we show that TIR-containing proteins catalyze production of 2'-(5''-phosphoribosyl)-5'-adenosine mono-/di-phosphate (pRib-AMP/ADP) in vitro and in planta. Biochemical and structural data demonstrate that EDS1-PAD4 is a receptor complex for pRib-AMP/ADP, which allosterically promote EDS1-PAD4 interaction with ADR1-L1 but not NRG1A. Our study identifies TIR-catalyzed pRib-AMP/ADP as a missing link in TIR signaling via EDS1-PAD4 and as likely second messengers for plant immunity.