Crystal structure of an insecticide-resistant acetylcholinesterase mutant (G119S) from the malaria vector Anopheles gambiae in complex with a difluoromethyl ketone inhibitor
Revelation date
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10-Jan-2018
Family
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ACHE: there are 543 genes in this family, 316 structure(s)
Title: Structure of the G119S Mutant Acetylcholinesterase of the Malaria Vector Anopheles gambiae Reveals Basis of Insecticide Resistance Cheung J, Mahmood A, Kalathur R, Liu L, Carlier PR Ref: Structure, 26:130, 2018 : PubMed
Malaria is a devastating disease in sub-Saharan Africa and is transmitted by the mosquito Anopheles gambiae. While indoor residual spraying of anticholinesterase insecticides has been useful in controlling the spread of malaria, widespread application of these compounds has led to the rise of an insecticide-resistant mosquito strain that harbors a G119S mutation in the nervous system target enzyme acetylcholinesterase. We demonstrate the atomic basis of insecticide resistance through structure determination of the G119S mutant acetylcholinesterase of An. gambiae in the ligand-free state and bound to a potent difluoromethyl ketone inhibitor. These structures reveal specific features within the active-site gorge distinct from human acetylcholinesterase, including an open channel at the base of the gorge, and provide a means for improving species selectivity in the rational design of improved insecticides for malaria vector control.
Representative scheme of ACHE structure and an image from PDBsum server
Databases
PDB-Sum
6ARY Previously Class, Architecture, Topology and Homologous superfamily - PDB-Sum server
FSSP
6ARYFold classification based on Structure-Structure alignment of Proteins - FSSP server
