Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.
Representative scheme of A85-Feruloyl-Esterase structure and an image from PDBsum server
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PDB-Sum
4H35 Previously Class, Architecture, Topology and Homologous superfamily - PDB-Sum server
FSSP
4H35Fold classification based on Structure-Structure alignment of Proteins - FSSP server
