The threat of a deliberate release of chemical nerve agents has underscored the need to continually improve field effective treatments for these types of poisonings. The oxime containing HLo-7 is a potential second-generation therapeutic reactivator. A synthetic process for HLo-7 is detailed with improvements to the DIBAL reduction and ion exchange steps. HLo-7 was visualized for the first time within the active site of human acetylcholinesterase and its relative ex vivo potency confirmed against various nerve agents using a phrenic nerve hemidiaphragm assay.
        
Representative scheme of ACHE structure and an image from PDBsum server
Databases
PDB-Sum
6NEA Previously Class, Architecture, Topology and Homologous superfamily - PDB-Sum server
FSSP
6NEAFold classification based on Structure-Structure alignment of Proteins - FSSP server