Title: Dipeptidyl peptidase-IV inhibitory activity of Katsuobushi-derived peptides in Caco-2 cell assay and oral glucose tolerance test in ICR mice Seki E, Yamamoto A, Fujiwara Y, Yamane T, Satsu H, Ohkubo I Ref: Journal of Agricultural and Food Chemistry, :, 2020 : PubMed
Proteolytic products of bonito stock residue inhibit dipeptidyl peptidase-IV (DPP-IV). Here, we isolated, purified, and identified the components of its N5 fraction obtained after using neutral protease from Aspergillus oryzae. A 10%-ethanol eluent (N5-2 fraction) from column chromatography was sequenced, yielding 18 peptides. Of these, Glu-Val-Phe, Ala-Va-Phe, and Gly-Val-Phe were identified as novel (IC50 values for DPP-IV inhibition were 525.56, 5466.49, and 2870.87 microM , respectively), whereas Trp-Val is the primary peptide (IC50 value of 36.99 microM, 1359 unit (mL/100g-N5-2 fraction) = (yield (mg/)/100g-N5-2 fraction)/ IC50(microg/mL). Furthermore, the N5-2 fraction significantly decreased DPP-IV activity in Caco-2 intestinal epithelial cells (p<0.05). From the oral glucose tolerance test using ICR mice, the N5-2 fraction significantly attenuated the rise in serum glucose levels compared with the control (p<0.05) through cell-surface DPP-IV inhibition. We discuss the novelty, the significance and relevance of finding study as well as its broad applications of prevention with diabetes.
Related information
Citations formats
Seki E, Yamamoto A, Fujiwara Y, Yamane T, Satsu H, Ohkubo I (2020) Dipeptidyl peptidase-IV inhibitory activity of Katsuobushi-derived peptides in Caco-2 cell assay and oral glucose tolerance test in ICR mice Journal of Agricultural and Food Chemistry
Seki E, Yamamoto A, Fujiwara Y, Yamane T, Satsu H, Ohkubo I (2020) Journal of Agricultural and Food Chemistry
