Paper Report for: Romano_2005_Tetrahedron.Asymmetry_16_841
Reference
Title: Enhanced enantioselectivity of Bacillus coagulans in the hydrolysis of 1,2-O-isopropylidene glycerol esters by thermal knock-out of undesired enzymes Romano D, Falcioni F, Mora D, Molinari F, Buthe A, Ansorge-Schumacher M Ref: Tetrahedron Asymmetry, 16:841, 2005 : PubMed
The enantioselective hydrolysis of different (RS)-1,2-O-isopropylidene glycerol esters has been achieved with whole cells of Bacillus coagulans NCIMB 9365 furnishing the (S)-alcohol as the major enantiomer. The reaction is catalysed by a thermostable cell-bound carboxylesterase and improvement of the enantioselectivity has been achieved by heat treatment of the whole cells, which causes the knock-outs a non-enantioselective competing enzyme. Thermally-treated cells hydrolysed (RS)-1,2-O-isopropylidene glycerol esters with high enantioselectivity, the highest enantiomeric ratio (80100) being observed for the benzoate. The biocatalyst displayed good stability and could be re-used after filtration for 12 cycles before showing significant loss of activity; repeated biotransformation batches allowed the recovery of 9.55 g/L of enantiomerically pure (S)-isopropylideneglycerol benzoate starting from 24.0 g/L of the racemic mixture.
Romano D, Falcioni F, Mora D, Molinari F, Buthe A, Ansorge-Schumacher M (2005) Enhanced enantioselectivity of Bacillus coagulans in the hydrolysis of 1,2-O-isopropylidene glycerol esters by thermal knock-out of undesired enzymes Tetrahedron Asymmetry16: 841-845
Romano D, Falcioni F, Mora D, Molinari F, Buthe A, Ansorge-Schumacher M (2005) Tetrahedron Asymmetry16: 841-845