Paper Report for: Ketterman_1989_Int.J.Biochem_21_1303
Reference
Title: Purification and characterization of two human liver carboxylesterases Ketterman AJ, Bowles MR, Pond SM Ref: International Journal of Biochemistry, 21:1303, 1989 : PubMed
1. Two carboxylesterases (EC 3.1.1.1) purified from human livers were distinguished by pI (isoelectric point), nondenaturing polyacrylamide gel electrophoresis, molecular weight, catalytic activity, N-terminus and immunological cross-reactivity. 2. The low pI carboxylesterase has not been reported previously. 3. Numerous bands seen when each enzyme was focused on analytical IEF gels could not be separated. 4. When sections of the band pattern was refocused, the original complete band pattern was generated. 5. Both the mid and low pI carboxylesterases had catalytic activity for xenobiotics as well as medium and long chain fatty acid esters.
        
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Ketterman AJ, Bowles MR, Pond SM (1989) Purification and characterization of two human liver carboxylesterases International Journal of Biochemistry21: 1303-12
Ketterman AJ, Bowles MR, Pond SM (1989) International Journal of Biochemistry21: 1303-12