Paper Report for: Hiraishi_2003_Biomacromolecules_4_80
Reference
Title: Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 from Sphingomonas sp. KT-1 Hiraishi T, Kajiyama M, Tabata K, Yamato I, Doi Y Ref: Biomacromolecules, 4:80, 2003 : PubMed
Sphingomonas sp. KT-1 hydrolyzes poly(aspartic acid) (PAA) containing alpha- and beta-amide units and has at least two different types of PAA hydrolases. The PAA hydrolase-1 hydrolyzes selectively beta-beta amide units in PAA. Molecular cloning of PAA hydrolase-1 from Sphingomonas sp. KT-1 has been carried out to characterize its gene products. Genetic analysis shows that the deduced amino acid sequence of PAA hydrolase-1 has a similarity with those of the catalytic domain of poly(3-hydroxybutyric acid) (PHB) depolymerases from Alcaligenes faecalis AE122 and Pseudomonas lemoignei. Site-specific mutation analysis indicates that (176)Ser is a part of a strictly conserved pentapeptide sequence (Gly-Xaa-Ser-Xaa-Gly), which is the lipase box, and plays as an active residue.
Hiraishi T, Kajiyama M, Tabata K, Yamato I, Doi Y (2003) Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 from Sphingomonas sp. KT-1 Biomacromolecules4: 80-86
Hiraishi T, Kajiyama M, Tabata K, Yamato I, Doi Y (2003) Biomacromolecules4: 80-86
