The C-terminal amidation of calcitonin represents an important technological problem. A method using a serine carboxypeptidase-catalyzed transpeptidation reaction in combination with photochemical cleavage to give the warranted peptide amide is described. The overall yield is higher than 95%.
Henriksen DB, Rolland M, Jakobsen MH, Buchardt O, Breddam K (1992) C-terminal amidation of calcitonin by carboxypeptidase Y catalyzed transpeptidation with a photocleavable nucleophile Pept Res5: 321-4
Henriksen DB, Rolland M, Jakobsen MH, Buchardt O, Breddam K (1992) Pept Res5: 321-4