Title: Investigation of lipase-catalysed hydrolysis of naproxen methyl ester: use of NMR spectroscopy methods to study substrate-enzyme interaction Cernia E, Delfini M, Di Cocco E, Palocci C, Soro S Ref: Bioorg Chem, 30:276, 2002 : PubMed
(+/-)-2-(6-Methoxy-2-naphthyl)propionic acid methyl ester (methyl ester of Naproxen), the precursor of therapeutically important nonsteroidal anti-inflammatory drugs (NSAIDs) was enantioselectively hydrolysed using as biocatalyst Candida rugosa lipase. In research aimed at studying the structure-activity relationship (SAR), NMR spectroscopy methods were employed to identify which Naproxen molecular moiety was essential to the substrate-enzyme interaction. The experimental results, in agreement with previous computer modelling studies and reported kinetic data, gave new information on the enzyme-substrate complex formation in solution.
Cernia E, Delfini M, Di Cocco E, Palocci C, Soro S (2002) Investigation of lipase-catalysed hydrolysis of naproxen methyl ester: use of NMR spectroscopy methods to study substrate-enzyme interaction Bioorg Chem30: 276-84
Cernia E, Delfini M, Di Cocco E, Palocci C, Soro S (2002) Bioorg Chem30: 276-84