Paper Report for: Aharoni_2004_Proc.Natl.Acad.Sci.U.S.A_101_482
Reference
Title: Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS Ref: Proc Natl Acad Sci U S A, 101:482, 2004 : PubMed
Serum paraoxonases (PONs) are a group of enzymes that play a key role in organophosphate (OP) detoxification and in prevention of atherosclerosis. However, their structure and mechanism of action are poorly understood. PONs seem like jacks-of-all-trades, acting on a very wide range of substrates, most of which are of no physiological relevance. Family shuffling and screening lead to the first PON variants that express in a soluble and active form in Escherichia coli. We describe variants with kinetic parameters similar to those reported for PONs purified from sera and others that show dramatically increased activities. In particular, we have evolved PON1 variants with OP-hydrolyzing activities 40-fold higher than wild type and a specificity switch of >2,000-fold, producing PONs specialized for OP rather than ester hydrolysis. Analysis of the newly evolved variants provides insights into the evolutionary relationships between different family members.
        
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Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS (2004) Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization Proc Natl Acad Sci U S A101: 482-7
Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS (2004) Proc Natl Acad Sci U S A101: 482-7