| Title : Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate - Pauwels_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_791 |
| Author(s) : Pauwels K , Loris R , Vandenbussche G , Ruysschaert JM , Wyns L , Van Gelder P |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 61 :791 , 2005 |
|
Abstract :
Bacterial lipases that are secreted via the type II secretion pathway require a lipase-specific foldase in order to obtain their native and biologically active conformation in the periplasmic space. The lipase-foldase complex from Burkholderia glumae (319 and 333 residues, respectively) was crystallized in two crystal forms. One crystal form belongs to space group P3(1)21 (P3(2)21), with unit-cell parameters a = b = 122.3, c = 98.2 A. A procedure is presented which improved the diffraction of these crystals from approximately 5 to 2.95 A. For the second crystal form, which belonged to space group C2 with unit-cell parameters a = 183.0, b = 75.7, c = 116.6 A, X-ray data were collected to 1.85 A. |
| PubMedSearch : Pauwels_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_791 |
| PubMedID: 16511160 |
| Gene_locus related to this paper: burgl-lipas |
| Gene_locus | burgl-lipas |
Pauwels K, Loris R, Vandenbussche G, Ruysschaert JM, Wyns L, Van Gelder P (2005)
Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate
Acta Crystallographica Sect F Struct Biol Cryst Commun
61 :791
Pauwels K, Loris R, Vandenbussche G, Ruysschaert JM, Wyns L, Van Gelder P (2005)
Acta Crystallographica Sect F Struct Biol Cryst Commun
61 :791