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Mutation Report for: V226I_human-ACHE

V226I_human-ACHE
Gene_Locus|human-ACHE
Mode of mutation|Site directed mutagenesis
Amino Acid change|V226I
Torpedo number|223
Summary|
Comment|p.V226I Val226Ile (p.V257I Val257Ile in primary sequence with 31 amino-acids signal peptide) mutation designed by -Protein Repair One Stop Shop- process for the production of active human acetylcholinesterase in a prokaryotic system. The mutation gives Ile as in Torpedo californica Bungar ACHE and human BCHE
Kinetic parameters|none


References:
    Title: Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability
    Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S and Fleishman SJ <6 more author(s)>
    Ref: Mol Cell, 63:337, 2016 : PubMed

            




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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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