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Mutation Report for: T284H/P285T/L286I/S287H/V288G_human-BCHE

Mode of mutation|Site directed mutagenesis
Amino Acid change|T284H/P285T/L286I/S287H/V288G
Torpedo number|286//287//288//289//290//286,287,288,289,290
Comment|p.T284H/P285T/L286I/S287H/V288G Thr284His/Pro285Thr/Leu286Ile/Ser287His/Val288Gly (p.T312H/P313T/L314I/S315H/V316G Thr312His/Pro313Thr/Leu314Ile/Ser315His/Val316Gly in primary sequence with 28 amino-acids signal peptide) Multiple amino acid substitutions in the acyl-binding loop 284-TPLSV-288 284-ThrProLeuSerVal-288 close to the active site. replaced by HisThrIleHisGly HTIHG clone 14. Slow self-reactivation after inhibition by paraoxon
Kinetic parameters|none

    Title: QM/MM Description of Newly Selected Catalytic Bioscavengers Against Organophosphorus Compounds Revealed Reactivation Stimulus Mediated by Histidine Residue in the Acyl-Binding Loop
    Zlobin A, Mokrushina Y, Terekhov S, Zalevsky A, Bobik T, Stepanova A, Aliseychik M, Kartseva O, Panteleev S and Smirnov I <3 more author(s)>
    Ref: Front Pharmacol, 9:834, 2018 : PubMed


    Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Terekhov SS, Smirnov IV, Stepanova AV, Bobik TV, Mokrushina YA, Ponomarenko NA, Belogurov AA, Jr., Rubtsova MP, Kartseva OV and Altman S <15 more author(s)>
    Ref: Proc Natl Acad Sci U S A, 114:2550, 2017 : PubMed


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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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