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Mutation Report for: N506S_ratno-Ces1d

N506S_ratno-Ces1d
Gene_Locus|ratno-Ces1d
Mode of mutation|Site directed mutagenesis
Amino Acid change|N506S
Torpedo number|481
Summary|
Comment|Increases specific activity against PNPC by 6-fold and against PNPA by 8.7- fold, no activity on cholesteryl oleate. Increases specific activity against PNPC by 7.5-fold and against PNPA by 3.6-fold, and increases cholesteryl esterase activity by 2.7 fold; when associated with I-423.
Kinetic parameters|none


References:
    Title: Mutation of residues 423 (Met/Ile), 444 (Thr/Met), and 506 (Asn/Ser) confer cholesteryl esterase activity on rat lung carboxylesterase. Ser-506 is required for activation by cAMP-dependent protein kinase
    Wallace TJ, Kodsi EM, Langston TB, Gergis MR, Grogan WM
    Ref: Journal of Biological Chemistry, 276:33165, 2001 : PubMed

            




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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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