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Mutation Report for: N303R/S332G_mouse-ABHD4

N303R/S332G_mouse-ABHD4
Gene_Locus|mouse-abhd4
Mode of mutation|Site directed mutagenesis
Amino Acid change|
Torpedo number|
Summary|
    Comment|Two highly conserved ABHD5 amino acids (R299 and G328) enabled ABHD4 (ABHD4 N303R/S332G) to activate ATGL in Cos7 cells, brown adipocytes, and artificial lipid droplets. The corresponding ABHD5 mutations (ABHD5 R299N and ABHD5 G328S) selectively disrupted lipolysis without affecting ATGL lipid droplet translocation or ABHD5 interactions with perilipin proteins and ABHD5 ligands
    Kinetic parameters|none


    References:
      Title: Molecular Basis of ABHD5 Lipolysis Activation
      Sanders MA, Zhang H, Mladenovic L, Tseng YY, Granneman JG
      Ref: Sci Rep, 7:42589, 2017 : PubMed

              




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