Kinetic parameters

Tree Display

AceDB Schema

XML Display


Mutation Report for: L153H_human-PLA2G7

Mode of mutation|Site directed mutagenesis
Amino Acid change|
Torpedo number|
    Comment|Tentative to engineer plasma platelet-activating factor acetylhydrolase by amino acid substitution into OP-hydrolyzing variants (bioscavengers). L153H, and F322H have essentially no hydrolytic activity against the nerve agents tested. In contrast, the W298H mutant displayed novel somanase activity with a kcat of 5min-1 and a KM of 590muM at pH7.5.
    Kinetic parameters|none

      Title: A rationally designed mutant of plasma platelet-activating factor acetylhydrolase hydrolyzes the organophosphorus nerve agent soman
      Kirby SD, Norris J, Sweeney R, Bahnson BJ, Cerasoli DM
      Ref: Biochimica & Biophysica Acta, 1854:1809, 2015 : PubMed


    Send your questions or comments to :
    Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
    Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
    For technical information about these pages see:
    ESTHER Home Page and ACEDB Home Page
    AcePerl Lincoln Stein Home Page

    Acknowledgements and disclaimer