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Mutation Report for: F227A/S287G/A328W/Y332M_human-BCHE

Mode of mutation|Site directed mutagenesis
Amino Acid change|F227A/S287G/A328W/Y332M
Torpedo number|229//289//330//334//229,289,330,334
Comment|p.F227A/S287G/A328W/Y332M Phe227Val/Ser287Gly/Ala328Trp/Tyr332Met (p.F255V/S315G/A356W/Y360G Phe255Val/Ser315Gly/Ala356Trp/Tyr360Gly in primary sequence with 28 amino-acids signal peptide) Cocaine hydrolysis;AME359 CocH, 34-fold improved catalytic efficiency against (-)-cocaine
Kinetic parameters|none

    Title: Effects of anti-cocaine vaccine and viral gene transfer of cocaine hydrolase in mice on cocaine toxicity including motor strength and liver damage
    Gao Y, Geng L, Orson FM, Kinsey B, Kosten TR, Shen X, Brimijoin S
    Ref: Chemico-Biological Interactions, 203:208, 2013 : PubMed


    Title: Cocaine hydrolase encoded in viral vector blocks the reinstatement of cocaine seeking in rats for 6 months
    Anker JJ, Brimijoin S, Gao Y, Geng L, Zlebnik NE, Parks RJ, Carroll ME
    Ref: Biological Psychiatry, 71:700, 2012 : PubMed


    Title: Cocaine hydrolase gene therapy for cocaine abuse
    Brimijoin S, Gao Y
    Ref: Future Med Chem, 4:151, 2012 : PubMed


    Title: Combined cocaine hydrolase gene transfer and anti-cocaine vaccine synergistically block cocaine-induced locomotion
    Carroll ME, Zlebnik NE, Anker JJ, Kosten TR, Orson FM, Shen X, Kinsey B, Parks RJ, Gao Y, Brimijoin S
    Ref: PLoS ONE, 7:e43536, 2012 : PubMed


    Title: The concept of pharmacologic cocaine interception as a treatment for drug abuse
    Gao Y, Orson FM, Kinsey B, Kosten T, Brimijoin S
    Ref: Chemico-Biological Interactions, 187:421, 2010 : PubMed


    Title: Design of high-activity mutants of human butyrylcholinesterase against (-)-cocaine: structural and energetic factors affecting the catalytic efficiency
    Zheng F, Yang W, Xue L, Hou S, Liu J, Zhan CG
    Ref: Biochemistry, 49:9113, 2010 : PubMed


    Title: Lasting reduction of cocaine action in neostriatum--a hydrolase gene therapy approach
    Gao Y, Brimijoin S
    Ref: Journal of Pharmacology & Experimental Therapeutics, 330:449, 2009 : PubMed


    Title: Gene transfer of cocaine hydrolase suppresses cardiovascular responses to cocaine in rats
    Gao Y, Atanasova E, Sui N, Pancook JD, Watkins JD, Brimijoin S
    Ref: Molecular Pharmacology, 67:204, 2005 : PubMed


    Title: Application of directed evolution technology to optimize the cocaine hydrolase activity of human butyrylcholinesterase
    Pancook JD, Pecht G, Ader M, Mosko M, Lockridge O, Watkins JD
    Ref: FASEB Journal, 17:A565, 2003 : PubMed


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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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