Mutation Report for: D74N/W286A_human-ACHE D74N/W286A_human-ACHE
Gene_Locus | human-ACHE Mode of mutation | Site directed mutagenesis Amino Acid change | D74N/W286A Torpedo number | 72,279 //279 //72 Summary | Comment | p.D74N/W286A Asp74Gln/Trp286Ala (p.D105N/W317A Asp105Gln/Trp317Ala in primary sequence with 31 amino-acids signal peptide) Peripheral Anionic Site;cooperativity of mutations D74 W286 10+4 increase Ki Kinetic parameters | none
References:
Title: Molecular Aspects of Catalysis and of Allosteric Regulation of AceytlcholinesterasesShafferman A , Ordentlich A , Barak D , Kronman C , Ariel N , Leitner M , Segall Y , Bromberg A , Reuveny S and Velan B <4 more author(s)> Shafferman A , Ordentlich A , Barak D , Kronman C , Ariel N , Leitner M , Segall Y , Bromberg A , Reuveny S , Marcus D , Bino T , Lazar A , Cohen S , Velan B (- 4) Ref: In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases, (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :189, 1995 : PubMed Abstract
