p.Asp273Glu This mutation is already found in different sequences of wild type strains
Kinetic parameters
|
none
References:
Title: Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae) Zhang Y, Guo M, Ma Z, You C, Gao X, Shi X Ref: Ecotoxicology, 29:35, 2020 : PubMed
Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene MdalphaE7 in the two strains revealed that four non-synonymous mutations (Trp(251)-Leu, Asp(273)-Glu, Ala(365)-Val, and Ile(396)-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked MdalphaE7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies.
