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Mutation Report for: A328W/Y332A_human-BCHE

Mode of mutation|Site directed mutagenesis
Amino Acid change|A328W/Y332A
Torpedo number|330//334//330,334
Comment|p.A328W/Y332A Ala328Trp/Tyr332Ala (p.A356W/Y360A Ala328Trp/Tyr332Ala in primary sequence with 28 amino-acids signal peptide) Cocaine hydrolysis;CocE 40-fold increased kcat and low increase in Km
Kinetic parameters|none

    Title: Design of high-activity mutants of human butyrylcholinesterase against (-)-cocaine: structural and energetic factors affecting the catalytic efficiency
    Zheng F, Yang W, Xue L, Hou S, Liu J, Zhan CG
    Ref: Biochemistry, 49:9113, 2010 : PubMed


    Title: Gene transfer of cocaine hydrolase suppresses cardiovascular responses to cocaine in rats
    Gao Y, Atanasova E, Sui N, Pancook JD, Watkins JD, Brimijoin S
    Ref: Molecular Pharmacology, 67:204, 2005 : PubMed


    Title: Visualizing viral transduction of a cocaine-hydrolyzing, human butyrylcholinesterase in rats
    Gao Y, Brimijoin S
    Ref: Chemico-Biological Interactions, 157-158:97, 2005 : PubMed


    Title: Molecular dynamics simulation of cocaine binding with human butyrylcholinesterase and its mutants
    Hamza A, Cho H, Tai HH, Zhan CG
    Ref: J Phys Chem B, 109:4776, 2005 : PubMed


    Title: Cocaine metabolism accelerated by a re-engineered human butyrylcholinesterase
    Sun H, Shen ML, Pang YP, Lockridge O, Brimijoin S
    Ref: Journal of Pharmacology & Experimental Therapeutics, 302:710, 2002 : PubMed


    Title: Re-engineering butyrylcholinesterase as a cocaine hydrolase
    Sun H, Pang YP, Lockridge O, Brimijoin S
    Ref: Molecular Pharmacology, 62:220, 2002 : PubMed


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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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