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Mutation Report for: A199S/F227A/S287G/A328W/Y332G/F364C/M532C_human-BCHE

Mode of mutation|Site directed mutagenesis
Amino Acid change|A199S/F227A/S287G/A328W/Y332G/F364C/M532C
Torpedo number|201//229//289//330//334//366//534//201,229,289,330,334,365,534
Comment|p.A199S/F227A/S287G/A328W/Y332G/F364C/M532C Ala199Ser/Phe227Ala/Ser287Gly/Ala328Trp/Tyr332Gly/Phe364Cys/M532Cys (p.A227S/F255A/S315G/A356W/Y360G/F392C/M560C Ala227Ser/Phe255Ala/Ser315Gly/Ala356Trp/Tyr360Gly/Phe392Cys/Met560Cys in primary sequence with 28 amino-acids signal peptide) denoted E364-532 for convenience. Mutations F364C/M532C introduce a new disulfide cross-subunit bond on the BChE mutated for increased cocaine hydrolysis
Kinetic parameters|none

    Title: Amino-acid mutations to extend the biological half-life of a therapeutically valuable mutant of human butyrylcholinesterase
    Fang L, Hou S, Xue L, Zheng F, Zhan CG
    Ref: Chemico-Biological Interactions, 214C:18, 2014 : PubMed


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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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