Kinetic parameters

Tree Display

AceDB Schema

XML Display


Mutation Report for: A199S/F227A/P285A/S287G/A328W/Y332G_human-BCHE

Mode of mutation|Site directed mutagenesis
Amino Acid change|A199S/F227A/P285A/S287G/A328W/Y332G
Torpedo number|201//229//287//289//330//334//201,229,287,289,330,334
Comment|p.A199S/F227A/P285A/S287G/A328W/Y332G Ala199Ser/Phe227Ala/Pro285Ala/Ser287Gly/Ala328Trp/Tyr332Gly (p.A227S/F255A/P313A/S315G/A356W/Y360G Ala227Ser/Phe255Ala/Pro313Ala/Ser315Gly/Ala356Trp/Tyr360Gly in primary sequence with 28 amino-acids signal peptide) denoted E30-6 for convenience Cocaine hydrolysis; kcat = 14600 min1, KM = 3.65 M, and kcat/KM = 4.0 109 min1 M1 for ()-cocaine hydrolysis in E30-6, whereas kcat = 8710 min1, KM = 54.7 M, and kcat/KM = 1.6 108 min1 M1 for ACh hydrolysis in E30-6
Kinetic parameters|none

    Title: Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose
    Li Y, Kong Q, Yue J, Gou X, Xu M, Wu X
    Ref: Nat Biomed Eng, 3:105, 2019 : PubMed


    Title: A highly efficient cocaine-detoxifying enzyme obtained by computational design
    Zheng F, Xue L, Hou S, Liu J, Zhan M, Yang W, Zhan CG
    Ref: Nat Commun, 5:3457, 2014 : PubMed


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page

Acknowledgements and disclaimer