Kinetic parameters for torpedo number 70,121,279,288,290,330

Substrates: Acetylthiocholine, Butyrylthiocholine

Inhibitors:

Substrates

Substrate: Acetylthiocholine

Gene_Locus	Enzyme	Km	Kcat	Exp. conditions	Reference
human-ACHE	Y72N/Y124Q/W286A/F295L/F297V/Y337A	4700 uM	0.8 10+5/min	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
bunfa-ACHE	WT	78.8 & 6.3 uM	-	T25C IS 50 mM phosphate	Cousin_1996_J.Biol.Chem_271_15099
chick-ACHE	WT	-	175000/min	100mM NaP T28C pH7.0	Vigny_1978_Eur.J.Biochem_85_317
eleel-ACHE	WT	130 uM	900000 /min		Salih_1992_Biochem.J_285_451
eleel-ACHE	WT	-	733333/min	100mM NaP T28C pH7.0	Vigny_1978_Eur.J.Biochem_85_317
human-ACHE	WT	120 uM	420000/min	50mM Na phosphate pH8	Barak_1994_J.Biol.Chem_269_6296
human-ACHE	WT	140 uM	4.0 10+5 /min	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
human-ACHE	WT	140 & 10 uM	400000 & 50000/min	50mM NaPhosphate pH8	Kronman_1994_J.Biol.Chem_269_27819
human-ACHE	WT	140 uM	370000/min	IS 50mM phosphate pH8 T27C	Ordentlich_1993_J.Biol.Chem_268_17083
human-ACHE	WT	140 uM	400000/min	5 or 50mM Na Phosphate pH8 T27C	Ordentlich_1995_J.Biol.Chem_270_2082
human-ACHE	WT	140 & 50 uM	370000 & 10000/min	pH8 T27C 50mM Na phosphate	Shafferman_1992_EMBO.J_11_3561
human-ACHE	WT	80 & 10 uM	400000 & 60000/min	IS 5mM	Shafferman_1994_EMBO.J_13_3448
human-ACHE	WT	117 uM	390000 /min	50mM Na phosphate pH8.0 T27C	Velan_1991_J.Biol.Chem_266_23977
human-BCHE	WT	40 uM	0.5 10+5 /min	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
mouse-ACHE	WT	46 uM	140000 /min	100mM NaPhosphate Ph7.0 T22C	Hosea_1995_Biochemistry_34_11528
mouse-ACHE	WT	46 & 3 uM	140000 & 10000/min	100mM NaP pH7 T22C eq3 sch3	Radic_1993_Biochemistry_32_12074
mouse-BCHE	WT	23 uM	40000 /min	100mM NaPhosphate Ph7.0 T22C	Hosea_1995_Biochemistry_34_11528
mouse-BCHE	WT	35 & 2 uM	40000 & 7000/min	100mM NaP pH7 T22C eq3 sch3	Radic_1993_Biochemistry_32_12074
ratno-ACHE	WT	-	220000/min	100mM NaP T28C pH7.0	Vigny_1978_Eur.J.Biochem_85_317
torca-ACHE	WT	-	481666/min	T25C pH7.8	Taylor_1974_Mol.Pharmacol_10_78
torma-ACHE	WT	81.2 & 4.2 uM	-	T25C IS 50 mM phosphate	Cousin_1996_J.Biol.Chem_271_15099
torma-ACHE	WT	-	273333/min	100mM NaP T28C pH7.0	Vigny_1978_Eur.J.Biochem_85_317
human-ACHE	WT	0.30 mM	-	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
human-ACHE	WT	2.5 mM	-	50mM NaPhosphate pH7.4	Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423

Substrate: Butyrylthiocholine

Gene_Locus	Enzyme	Km	Kcat	Exp. conditions	Reference
human-ACHE	Y72N/Y124Q/W286A/F295L/F297V/Y337A	3.3 mM	1.5 10+5/min	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
human-ACHE	WT	0.30 mM	0.08 10+5/min	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
human-ACHE	WT	2.5 mM	70000 /min	50mM NaPhosphate pH7.4	Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
human-BCHE	WT	7 & 0.7 uM	24000 & 10000 /min	100mM K phosphate pH7.0 T25C	Blong_1997_Biochem.J_327_747
human-BCHE	WT	2.2 mM	-	100mM Na phosphate pH7.4 T22C	Gnatt_1994_J.Neurochem_62_749
human-BCHE	WT	0.05 mM	1.1 10+5 /min	IS 50mM phosphate pH8 T27C	Kaplan_2001_Biochemistry_40_7433
human-BCHE	WT	2.8 & 0.7 mM	98000 & 33000 /min	50mM NaPhosphate pH7.4	Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
mouse-ACHE	WT	93 uM	1100 /min	100mM NaPhosphate Ph7.0 T22C	Hosea_1995_Biochemistry_34_11528
mouse-BCHE	WT	55 uM	37000 /min	100mM NaPhosphate Ph7.0 T22C	Hosea_1995_Biochemistry_34_11528

Inhibitors