Torpedo_number

Kinetic parameters

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Kinetic parameters for torpedo number 351 for human-ACHE

Substrates: 3,3-dimethylbutylthioacetate, Acetylthiocholine

Inhibitors: Edrophonium

Substrates

Substrate: 3,3-dimethylbutylthioacetate
EnzymeKmKcatExp. conditionsReference
D349N/E358Q/E389Q/D390N 240 & 40 uM 49000 & 8000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
D349N/E358Q 200 & 30 uM 38000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E292A/D349N/E358Q/E389Q/D390N 250 & 40 uM 47000 & 7000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E292A/D349N/E358Q 260 & 40 uM 47000 & 7000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E358Q 230 & 40 uM 45000 & 7000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/D349N/E358Q/E389Q/D390N 230 & 40 uM 42000 & 5000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/D349N/E358Q 290 & 50 uM 48000 & 7000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E285A/E292A/D349N/E358Q/E389Q/D390N 220 & 30 uM 44000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E285A/E292A/D349N/E358Q 220 & 30 uM 40000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E292A/D349N/E358Q/E389Q/D390N 200 & 30 uM 42000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E292A/D349N/E358Q 260 & 30 uM 46000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
WT 300 & 6 mM 55000 & 10000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 450 uM 52000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 300 uM 55000/min 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
WT 260 & 30 uM 46000 & 7000/min IS 5mM Shafferman_1994_EMBO.J_13_3448

Substrate: Acetylthiocholine
EnzymeKmKcatExp. conditionsReference
D349N/E358Q/E389Q/D390N 70 & 10 uM 360000 & 50000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
D349N/E358Q 80 & 10 uM 380000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E292A/D349N/E358Q/E389Q/D390N 90 & 10 uM 400000 & 70000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E292A/D349N/E358Q 90 & 10 uM 400000 & 50000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E358Q 80 & 10 uM 360000 & 50000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/D349N/E358Q/E389Q/D390N 160 & 20 uM 360000 & 50000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/D349N/E358Q 200 & 30 uM 430000 & 70000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E285A/E292A/D349N/E358Q/E389Q/D390N 190 & 30 uM 380000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E285A/E292A/D349N/E358Q 210 & 30 uM 400000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E292A/D349N/E358Q/E389Q/D390N 190 & 30 uM 390000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E292A/D349N/E358Q 200 & 30 uM 390000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
WT 120 uM 420000/min 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
WT 140 uM 4.0 10+5 /min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
WT 140 & 10 uM 400000 & 50000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 140 uM 370000/min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 140 uM 400000/min 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
WT 140 & 50 uM 370000 & 10000/min pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561
WT 80 & 10 uM 400000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
WT 117 uM 390000 /min 50mM Na phosphate pH8.0 T27C Velan_1991_J.Biol.Chem_266_23977
WT 0.30 mM - IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
WT 2.5 mM - 50mM NaPhosphate pH7.4 Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423


Inhibitors

Inhibitor: Edrophonium
EnzymeKiKsiIc50SubstrateExp. conditionsReference
D349N/E358Q/E389Q/D390N 0.80 & 0.12 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
D349N/E358Q 0.90 & 0.14 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E292A/D349N/E358Q/E389Q/D390N 0.70 & 0.11 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E292A/D349N/E358Q 0.80 & 0.12 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E358Q 0.50 & 0.08 uM - - Acetylthiocholine IS 150mM Shafferman_1994_EMBO.J_13_3448
E84Q/D349N/E358Q/E389Q/D390N 1.70 & 0.24 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E84Q/D349N/E358Q 1.80 & 0.30 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E84Q/E285A/E292A/D349N/E358Q/E389Q/D390N 1.40 & 0.20 uM - - Acetylthiocholine IS 5mM Shafferman_1994_EMBO.J_13_3448
E84Q/E285A/E292A/D349N/E358Q 1.70 & 0.25 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E84Q/E292A/D349N/E358Q/E389Q/D390N 1.70 & 0.26 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
E84Q/E292A/D349N/E358Q 1.60 & 0.24 uM - - Acetylthiocholine IS 150 mM Shafferman_1994_EMBO.J_13_3448
WT 0.6 & 0.1 uM - - 3.3-dimethylbutylthioacetate 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 0.75 uM - - Acetylthiocholine 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
WT 50 uM - - Acetylthiocholine T25C IS 50 mM phosphate Kaplan_2001_Biochemistry_40_7433
WT 0.7 & 0.1 uM - - Acetylthiocholine 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
WT 0.3 uM - - Acetylthiocholine IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 0.6 uM 4.8 uM - Acetylthiocholine 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
WT - - 1.5 uM Acetylthiocholine pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561
WT 0.32 & 0.05 uM - - Acetylthiocholine IS 5mM Shafferman_1994_EMBO.J_13_3448
WT 0.4 uM - - Paranitrophenylacetate IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
WT 0.24 uM - - S-N-Propylthioacetate IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083

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