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Torpedo_number
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Kinetic parameters for torpedo number 290 for mouse-ACHE
Substrates:
Acetylthiocholine
,
Benzoylthiocholine
,
Butyrylthiocholine
Inhibitors:
WT-fasciculin2
Substrates
Substrate
:
Acetylthiocholine
Enzyme
Km
Kcat
Exp. conditions
Reference
F295L/F297I
200 & 93 uM
12000 & 1000/min
100mM NaP pH7 T22C eq3 sch3
Radic_1993_Biochemistry_32_12074
F297A
61 uM
61000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297I
170 uM
15000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297I
172 & 64 uM
15000 & 2000/min
100mM NaP pH7 T22C eq3 sch3
Radic_1993_Biochemistry_32_12074
F297Y
58 uM
39000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297Y
58 & 6 uM
39000 & 13000/min
100mM NaP pH7 T22C eq3 sch3
Radic_1993_Biochemistry_32_12074
WT
46 uM
140000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
WT
46 & 3 uM
140000 & 10000/min
100mM NaP pH7 T22C eq3 sch3
Radic_1993_Biochemistry_32_12074
Substrate
:
Benzoylthiocholine
Enzyme
Km
Kcat
Exp. conditions
Reference
F297A
4.8 uM
130 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297I
19 uM
450 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297Y
13 uM
80 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
WT
46 uM
30 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
Substrate
:
Butyrylthiocholine
Enzyme
Km
Kcat
Exp. conditions
Reference
F297A
86 uM
17000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297I
82 uM
60000 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
F297Y
45 uM
1800 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
WT
93 uM
1100 /min
100mM NaPhosphate Ph7.0 T22C
Hosea_1995_Biochemistry_34_11528
Inhibitors
Inhibitor
:
WT-fasciculin2
Enzyme
Ki
Ksi
Ic50
Substrate
Exp. conditions
Reference
F297I
-
19 pM
-
Acetylthiocholine
100mM NaP pH7.0 T37C eq4 sch4 meth5
Radic_1994_J.Biol.Chem_269_11233
F297Y
-
6.6 pM
-
Acetylthiocholine
100mM NaP pH7.0 T37C eq4 sch4 meth5
Radic_1994_J.Biol.Chem_269_11233
WT
-
2.3 & 0.6 pM
-
Acetylthiocholine
50mM NaP pH8.0
Marchot_1997_J.Biol.Chem_272_3502
WT
-
6.3 pM
-
Acetylthiocholine
100mM NaP pH7.0 T37C eq4 sch4 meth5
Radic_1994_J.Biol.Chem_269_11233
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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