Torpedo_number

Kinetic parameters

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Kinetic parameters for torpedo number 290 for mouse-ACHE

Substrates: Acetylthiocholine, Benzoylthiocholine, Butyrylthiocholine

Inhibitors: WT-fasciculin2

Substrates

Substrate: Acetylthiocholine
EnzymeKmKcatExp. conditionsReference
F295L/F297I 200 & 93 uM 12000 & 1000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074
F297A 61 uM 61000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297I 170 uM 15000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297I 172 & 64 uM 15000 & 2000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074
F297Y 58 uM 39000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297Y 58 & 6 uM 39000 & 13000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074
WT 46 uM 140000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
WT 46 & 3 uM 140000 & 10000/min 100mM NaP pH7 T22C eq3 sch3 Radic_1993_Biochemistry_32_12074

Substrate: Benzoylthiocholine
EnzymeKmKcatExp. conditionsReference
F297A 4.8 uM 130 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297I 19 uM 450 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297Y 13 uM 80 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
WT 46 uM 30 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528

Substrate: Butyrylthiocholine
EnzymeKmKcatExp. conditionsReference
F297A 86 uM 17000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297I 82 uM 60000 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
F297Y 45 uM 1800 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528
WT 93 uM 1100 /min 100mM NaPhosphate Ph7.0 T22C Hosea_1995_Biochemistry_34_11528


Inhibitors

Inhibitor: WT-fasciculin2
EnzymeKiKsiIc50SubstrateExp. conditionsReference
F297I - 19 pM - Acetylthiocholine 100mM NaP pH7.0 T37C eq4 sch4 meth5 Radic_1994_J.Biol.Chem_269_11233
F297Y - 6.6 pM - Acetylthiocholine 100mM NaP pH7.0 T37C eq4 sch4 meth5 Radic_1994_J.Biol.Chem_269_11233
WT - 2.3 & 0.6 pM - Acetylthiocholine 50mM NaP pH8.0 Marchot_1997_J.Biol.Chem_272_3502
WT - 6.3 pM - Acetylthiocholine 100mM NaP pH7.0 T37C eq4 sch4 meth5 Radic_1994_J.Biol.Chem_269_11233

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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