Kinetic parameters for mutation Y337F_mouse-ACHE

Molecule	Km	Kcat	Exp. conditions	Reference
Acetylthiocholine	53 & 5 uM	68000 & 26000/min	100mM NaP pH7 T22C eq3 sch3	Radic_1993_Biochemistry_32_12074

Inhibitor

Molecule	Ki	Ksi	Ic50	Exp. conditions	Substrate	Reference
9-Aminoacridine	171 & 55 nM	710 & 150 nM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Acridine	20 & 4 uM	210 & 22 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Chlorpromazine	22 & 11 uM	540 & 191 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Edrophonium	0.29 & 0.06 uM	7.8 & 0.8 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Ethopropazine	34 & 6 uM	330 & 86 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
N-Methyl-acridinium	1.3 & 0.3 uM	7.8 & 2.3 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Promazine	51 & 12 uM	850 & 21 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Promethazine	110 & 20 uM	1700 & 440 uM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074
Tacrine	150 & 30 nM	680 & 180 nM	-	100mM NaP pH7.0 T22C	Acetylthiocholine	Radic_1993_Biochemistry_32_12074

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page