Mutation

Kinetic parameters

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Kinetic parameters for mutation W86A_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
3,3-dimethylbutylthioacetate 440 & 70 uM 18000 & 1400/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
3,3-dimethylbutylthioacetate 550 uM 28000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
3,3-dimethylbutylthioacetate 440 uM 18000/min 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
Acetylthiocholine 93000 uM 80000/min 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
Acetylthiocholine 94000 & 15000 uM 80000 & 10000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
Acetylthiocholine 93300 uM 80000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
Acetylthiocholine 94000 uM 80000/min 5 or 50mM Na Phosphate pH8 T27C Ordentlich_1995_J.Biol.Chem_270_2082
Paranitrophenylacetate 3000 uM 190000 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083
S-N-Propylthioacetate 11500 uM 1400 /min IS 50mM phosphate pH8 T27C Ordentlich_1993_J.Biol.Chem_268_17083

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 40000 nM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Decamethonium 90000 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Decamethonium 90000 uM 30000 uM - 5 or 50mM Na Phosphate pH8 T27C Acetylthiocholine Ordentlich_1995_J.Biol.Chem_270_2082
Edrophonium >150 uM - - 3-dimethylbutylthioacetate 3 IS 50mM phosphate pH8 T27C
Edrophonium >45000 uM - - 50mM NaPhosphate pH8 3.3-dimethylbutylthioacetate Kronman_1994_J.Biol.Chem_269_27819
Edrophonium >150 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Edrophonium >45000 uM - - 50mM NaPhosphate pH8 Acetylthiocholine Kronman_1994_J.Biol.Chem_269_27819
Edrophonium >150 uM - - IS 50mM phosphate pH8 T27C Acetylthiocholine Ordentlich_1993_J.Biol.Chem_268_17083
Edrophonium >45000 uM - - 5 or 50mM Na Phosphate pH8 T27C Acetylthiocholine Ordentlich_1995_J.Biol.Chem_270_2082
Edrophonium >150 uM - - IS 50mM phosphate pH8 T27C Paranitrophenylacetate Ordentlich_1993_J.Biol.Chem_268_17083
Edrophonium >150 uM - - IS 50mM phosphate pH8 T27C S-N-Propylthioacetate Ordentlich_1993_J.Biol.Chem_268_17083
Hexamethonium 144.6 mM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
HuperzineA >1000000 nM - - 50mM Na phosphate pH8 T22C Acetylthiocholine Ariel_1998_Biochem.J_335_95
Propidium 870 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Propidium 870 uM 13.5 uM - 5 or 50mM Na Phosphate pH8 T27C Acetylthiocholine Ordentlich_1995_J.Biol.Chem_270_2082
Tacrine 1500000 & 400000 nM - - 50mM Na phosphate pH8 T22C Acetylthiocholine Ariel_1998_Biochem.J_335_95

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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