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Kinetic parameters for mutation W86A_human-ACHE
Substrate
Molecule
Km
Kcat
Exp. conditions
Reference
3,3-dimethylbutylthioacetate
440 & 70 uM
18000 & 1400/min
50mM NaPhosphate pH8
Kronman_1994_J.Biol.Chem_269_27819
3,3-dimethylbutylthioacetate
550 uM
28000 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
3,3-dimethylbutylthioacetate
440 uM
18000/min
5 or 50mM Na Phosphate pH8 T27C
Ordentlich_1995_J.Biol.Chem_270_2082
Acetylthiocholine
93000 uM
80000/min
50mM Na phosphate pH8
Barak_1994_J.Biol.Chem_269_6296
Acetylthiocholine
94000 & 15000 uM
80000 & 10000/min
50mM NaPhosphate pH8
Kronman_1994_J.Biol.Chem_269_27819
Acetylthiocholine
93300 uM
80000 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
Acetylthiocholine
94000 uM
80000/min
5 or 50mM Na Phosphate pH8 T27C
Ordentlich_1995_J.Biol.Chem_270_2082
Paranitrophenylacetate
3000 uM
190000 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
S-N-Propylthioacetate
11500 uM
1400 /min
IS 50mM phosphate pH8 T27C
Ordentlich_1993_J.Biol.Chem_268_17083
Inhibitor
Molecule
Ki
Ksi
Ic50
Exp. conditions
Substrate
Reference
BW284C51
40000 nM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Decamethonium
90000 uM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Decamethonium
90000 uM
30000 uM
-
5 or 50mM Na Phosphate pH8 T27C
Acetylthiocholine
Ordentlich_1995_J.Biol.Chem_270_2082
Edrophonium
>150 uM
-
-
3-dimethylbutylthioacetate
3
IS 50mM phosphate pH8 T27C
Edrophonium
>45000 uM
-
-
50mM NaPhosphate pH8
3.3-dimethylbutylthioacetate
Kronman_1994_J.Biol.Chem_269_27819
Edrophonium
>150 uM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Edrophonium
>45000 uM
-
-
50mM NaPhosphate pH8
Acetylthiocholine
Kronman_1994_J.Biol.Chem_269_27819
Edrophonium
>150 uM
-
-
IS 50mM phosphate pH8 T27C
Acetylthiocholine
Ordentlich_1993_J.Biol.Chem_268_17083
Edrophonium
>45000 uM
-
-
5 or 50mM Na Phosphate pH8 T27C
Acetylthiocholine
Ordentlich_1995_J.Biol.Chem_270_2082
Edrophonium
>150 uM
-
-
IS 50mM phosphate pH8 T27C
Paranitrophenylacetate
Ordentlich_1993_J.Biol.Chem_268_17083
Edrophonium
>150 uM
-
-
IS 50mM phosphate pH8 T27C
S-N-Propylthioacetate
Ordentlich_1993_J.Biol.Chem_268_17083
Hexamethonium
144.6 mM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
HuperzineA
>1000000 nM
-
-
50mM Na phosphate pH8 T22C
Acetylthiocholine
Ariel_1998_Biochem.J_335_95
Propidium
870 uM
-
-
50mM Na phosphate pH8
Acetylthiocholine
Barak_1994_J.Biol.Chem_269_6296
Propidium
870 uM
13.5 uM
-
5 or 50mM Na Phosphate pH8 T27C
Acetylthiocholine
Ordentlich_1995_J.Biol.Chem_270_2082
Tacrine
1500000 & 400000 nM
-
-
50mM Na phosphate pH8 T22C
Acetylthiocholine
Ariel_1998_Biochem.J_335_95
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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